THE ROLE OF CYP4502E1 GENETIC POLYMORPHISM ON BENZEN E METABOLISM

Author :Dewi Sumaryani Soemarko

Seminat :Okupasi

Journal : PERDOKI

Abstract :

Benzene has genotoxic/carcinogenic characteristic which always make it interesting subject for research. The carcinogenic effect is also associated with metabolites produced in benzene metabolism such as phenol, catechol, quinones, muconic acid (tt-MA), and phenyl mercapturic acid (s-PMA).

The role of CYP4502E1 enzyme in benzene metabolism is very important, which is determined by its genetic polymorphism. The presented cross-sectional study was aimed to obtain the distribution of frequency of s-PMA concentration in workers who had been low exposure of benzene based on CYP4502E1 genetic polymorphism. The study was conducted as a part of another study, i.e. the effect of low -level benzene exposure on the incidence of chromosomal breakage in 115 full-time workers at an oil company in Kalimantan between September 2007 and April 2010. Data were collected by methods of interviews, physical examinations, laboratory examinations, and direct observation on the work place. The variables studied were CYP4502E1, benzene exposure at the work place, age, type of work, history of work, length of work, body mass index (BMI), antioxidants intake, behavior and management, and s-PMA concentration in the urine. The distribution of CYP4502E1 genetic polymorphism in workers is 87.8% wild type homozygote, 11.3% heterozygote and 0.9% mutant homozygote. There was no difference in the proportion of s-PMA concentration based on CYP4502E1 genetic polymorphism (p=0.595; ORraw=0.98; 95% CI=0.95-1.01). There were also no differences of age, type of work, length of work, BMI, antioxidants consumptions, behavior and management of subjects with s-PMA. Further study should be performed on CYP4502E1 genetic polymor phism in various Indonesian races at different workplace with low-level benzene.

Key Words: low-level benzene exposure, CYP4502E1, s-PMA

 

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PERHIMPUNAN SPESIALIS KEDOKTERAN OKUPASI INDONESIA

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